| 质粒名称: | lenti-sgRNA blast |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 104993 |
| 存储实验室: | Brett Stringer |
|
载体骨架基本信息 |
|
| 载体名称: | lentiCRISPRv2 blast (Addgene #98293) |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 10437bp |
| 载体类型: | 哺乳细胞表达, Lentiviral, CRISPR |
| 筛选标记: | Blasticidin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl4 |
| 拷贝数: | 高拷贝 |
|
文献引用方法 |
|
| 材料和方法部分: | lenti-sgRNA blast was a gift from Brett Stringer (Addgene plasmid # 104993 ; http://n2t.net/addgene:104993 ; RRID:Addgene_104993) |
| 参考文献部分: | A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis PL, Jeffree RL, Johns TG, Boyd AW. Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. 10.1038/s41598-019-41277-z PubMed 30894629 |
买家导航
lenti-sgRNA blast载体质粒基本信息
lenti-sgRNA blast质粒图谱载体图谱和lenti-sgRNA blast载体序列质粒序列多克隆位点信息
lenti-sgRNA blast质粒载体简介
lenti-sgRNA blast质粒序列载体序列
LOCUS lenti-sgRNA blast 10437 bp DNA circular SYN 14-APR-2025
DEFINITION This 3rd generation lentiviral plasmid expresses a S. pyogenes
CRISPR chimeric RNA element with customizable sgRNA from a U6
promoter and blasticidin S resistance from an EF-1a promoter..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10437)
AUTHORS Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC,
Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T,
Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis
PL, Jeffree RL, Johns TG, Boyd AW
TITLE A reference collection of patient-derived cell line and xenograft
models of proneural, classical and mesenchymal glioblastoma.
JOURNAL Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
PUBMED 30894629
REFERENCE 2 (bases 1 to 10437)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep.";
date: "2019-03-20"; volume: "9(1):4902. doi"; pages: "
10.1038/s41598-019-41277-z"
COMMENT Sequence Label: lenti-sgRNA blast
FEATURES Location/Qualifiers
source 1..10437
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 9..30
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(149..169)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 154..483
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 334..469
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 396..415
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 531..578
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 597..971
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 1101..1222
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1138..1157)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1192..1211
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(1271..1287)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1271..1287)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1284..1306)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1295..1311
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(1319..1325,1326..1343,1344..1349))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1364..1385
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1502..1519)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1673..2261)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1753..1772)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(2432..3223,3224..3292))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3055..3074
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3293..3397)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3472..3491)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3667..4046
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4048..4246
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4201..4221
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 4264..4444
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4491..4616
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5109..5342
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 5527..5571
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 5869..5986
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 6037..6285
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
primer_bind 6037..6057
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 6208..6227
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 8167..8242
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 8304..8515
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 8534..8543
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 8540..8938
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 8954..9542
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(9007..9027)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(9425..9436)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 9614..9847
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(9873..9890)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9879..10103
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin join(10149..10437,1..140)
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(10236..10255)
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
61 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
121 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
301 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
361 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
421 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
481 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
541 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
601 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
661 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
721 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
781 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
841 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
901 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
961 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
1021 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
1081 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
1141 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
1201 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
1261 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
1321 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
1381 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
1441 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
1501 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
1561 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
1621 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
1681 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
1741 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
1801 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
1861 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
1921 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
1981 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
2041 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
2101 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
2161 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
2221 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
2281 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
2341 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
2401 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
2461 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
2521 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
2581 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
2641 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
2701 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
2761 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
2821 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
2881 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
2941 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
3001 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
3061 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
3121 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
3181 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
3241 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
3301 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
3361 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
3421 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
3481 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
3541 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
3601 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
3661 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
3721 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
3781 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
3841 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
3901 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
3961 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
4021 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
4081 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
4141 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
4201 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
4261 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
4321 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
4381 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
4441 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
4501 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
4561 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
4621 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
4681 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
4741 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
4801 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
4861 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
4921 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
4981 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
5041 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
5101 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
5161 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
5221 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
5281 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
5341 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
5401 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
5461 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
5521 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
5581 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
5641 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
5701 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
5761 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
5821 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
5881 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
5941 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
6001 ggacagcaga gatccagttt ggttaattaa ggtaccgagg gcctatttcc catgattcct
6061 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
6121 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
6181 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
6241 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaga cggttgtaaa
6301 tgagcacaca aaatacacat gctaaaatat tatattctat gacctttata aaatcaacca
6361 aaatcttctt tttaataact ttagtatcaa taattagaat ttttatgttc ctttttgcaa
6421 acttttaata aaaatgagca aaataaaaaa acgctagttt tagtaactcg cgttgttttc
6481 ttcaccttta ataatagcta ctccaccact tgttcctaag cggtcagctc ctgcttcaat
6541 cattttttga gcatcttcaa atgttctaac tccaccagct gctttaacta aagcattgtc
6601 tttaacaact gacttcatta gtttaacatc ttcaaatgtt gcacctgatt ttgaaaatcc
6661 tgttgatgtt ttaacaaatt ctaatccagc ttcaacagct atttcacaag ctttcatgat
6721 ttcttctttt gttaataaac aattttccat aatacattta acaacatgtg atccagctgc
6781 tttttttaca gctttcatgt cttctaaaac taattcataa tttttgtctt ttaatgcacc
6841 aatatttaat accatatcaa tttctgttgc accatcttta attgcttcag aaacttcgaa
6901 tgcttttgta gctgttgtgc atgcacctag aggaaaacct acaacatttg ttattcctac
6961 atttgtgcct tttaataatt ctttacaata gcttgttcaa tatgaattaa cacaaactgt
7021 tgcaaaatca aattcaattg cttcatcaca taattgttta atttcagctt tcgtagcatc
7081 ttgttttaat aatgtgtgat ctatatattt gtttagtttc attttttctc ctatatattc
7141 atttttaatt ttaattcttt aataatttcg tctactttaa ctttagcgtt ttgaacagat
7201 tcaccaacac ctataaaata aatttttagt ttaggttcag ttccacttgg gcgaacagca
7261 aatcatgact tatcttctaa ataaaatttt agtaagtctt gtcctggcat attatacatt
7321 ccatcgatgt agtcttcaac attaacaact ttaagtccag caatttgagt taagggtgtt
7381 gctctcaatg atttcattaa tggttcaatt tttaatttct tttcttctgg tttaaaattc
7441 aagtttaaag tgaaagtgta atatgcaccc atttctttaa ataaatcttc taaatagtct
7501 actaatgttt tattttgttt tttataaaat caagcagcct ctgctattaa tatagaagct
7561 tgtattccat ctttatctct agctgagtca tcaattacat atccataact ttcttcataa
7621 gcaaaaacaa aatttaatcc gttatcttct tctttagcaa tttctctacc cattcattta
7681 aatccagtta aagtttttac aatattaact ccatattttt catgagcgat tctatcaccc
7741 aaatcacttg ttacaaaact tgaatataga gccggatttt ttggaatgct atttaagcgt
7801 tttagatttg ataattttca atcaattaaa attggtcctg tttgatttcc atctaatctt
7861 acaaaatgac catcatgttt tattgccatt ccaaatctgt cagcatctgg gtcattcata
7921 ataataatat ctgcatcatg tttaatacca tattcaagcg gtatttttca tgcaggatca
7981 aattctggat ttggatttac aacattttta aatgtttcat cttcaaatgc atgctcttca
8041 acctcaataa cgttatatcc tgattcacgt aatatttttg gggtaaattt agttcctgtt
8101 ccattaactg cgctaaaaat aatttttaaa tcttttttag cttcttgctc ttttttgtac
8161 gtctctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
8221 aaaagtggca ccgagtcggt gcttttttga attcgctagc taggtcttga aaggagtggg
8281 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
8341 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
8401 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
8461 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggaccg
8521 gttctagagc gctgccacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
8581 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
8641 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
8701 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
8761 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
8821 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
8881 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaaac
8941 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
9001 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
9061 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
9121 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
9181 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
9241 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
9301 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
9361 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
9421 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
9481 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
9541 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
9601 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
9661 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
9721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
9781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
9841 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
9901 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
9961 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
10021 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
10081 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
10141 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
10201 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
10261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
10321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
10381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgtt
//
